Cells & tissues analyses
SINGLE CELL ANALYSIS
Single cell platform activities are based on droplet-based-microfluidics. This technology consists in compartmentalizing a sample in droplets at less than a cell per compartment/droplet. Biological analyses are thus individual at the single cell level. This technology dramatically reduces assay trials time, reactions volume (up to a million times compared to conventional tubes or plates assays) and costs.
Another advantage of individualizing reactions at the single cell level is to take cell diversity into account. Indeed, the very high sensitivity of the analyses in droplets allows analysing both the cells majority and the few rare cells of the sample.
Key Information:
- 10X Genomics Chromium 10X for ready for use Single Cell RNA Sequencing experiments
- The inDrop System instrument of 1cellBio to do Single Cell RNA sequencing experiments
- An evolutionary microfluidic station
CYTOMETRY PLATFORM INSTITUT CURIE - CYTPIC
The platform's mission is to guide and support scientific projects by offering a range of services ranging from empowerment through training, assistance on the various instruments, help in the construction of experiments to optimization of panels and collaboration, including the analysis of data generated. In order to cover a plurality of research axes, it offers a multitude of technologies: Conventional (analysis and cell sorting), Spectral (analysis and cell sorting) and Spatial Cytometry for multiplex tissue analysis with single cell resolution; we also provide instruments for circulating biomarker detection up to 100 biomolecules, tissue staining and analysis, cellular barcoding for transcriptomic, proteomic and/or epigenetic analysis.
Key information :
- Instruments available over 3 sites (Paris, Orsay and St-Cloud);
- Spectral Spatial Cytometer for multiplex tissue analysis, with single cell resolution;
- Transversal skills: biomarkers and tissue analysis, spectral and image cytometry, supervised and unsupervised data analysis.
THE BIOIMAGING CELL AND TISSUE CORE FACILITY
The Bioimaging Cell and Tissue Core Facility of Institut Curie (PICT-IBiSA) gathers highly sophisticated equipment and up to date technologies in advanced microscopy and image optimization/analysis. The objective of this imaging platform is to provide to your company a full offer to characterize every type of tissue with the resolution needed. The platform is a member of FranceBioImaging consortium and is labeled as an IBISA quality platform.
Optical microscopy
Key information:
- All together the equipment in light microscopy comprises in 2018 more than 40 systems:
- 7 confocal laser scanning microscopes (2 equipped with femtosecond pulsed laser for multiphoton excitation)
- 8 confocal spinning-disk systems (some equipped with laser photo-perturbation modules)
- 8 video-microscopes
- 3 3D deconvolution microscopes
- 2 TIRF systems (with FRAP or PALM techniques)
- 2 FLIM systems (TCSPC and phase modulation)
- 2 high-resolution structured illumination microscopes (OMX and Nikon HR-SIM)
- 2 optical sectioning Zeiss Apotome systems and several standard widefield microscopes for routine observation or histology.
Electron microscopy
Key information:
- Cellular 2D and 3D (tomography) electron microscopy of resin-embebded samples
- Cellular sample preparation (resin-embedded, immunolabelling, Tokuyasu)
- 2D and 3D cryo-electron microscopy of biomimetic samples
- 2D and 3D electron microscopy of negatively stained samples
Chemical imaging
The chemical imaging platform provide a qualitative and semiquantitative information on the distribution of chemical elements on a given sample.
Key information:
- Ion mass spectroscopy imaging
- Electron Energy Filtered TEM or EFTEM
- Electron tomography
- Chemical mapping by TEM.
